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1.
Mem. Inst. Oswaldo Cruz ; 110(4): 569-572, 09/06/2015. graf
Article in English | LILACS | ID: lil-748856

ABSTRACT

In the early 2015, several cases of patients presenting symptoms of mild fever, rash, conjunctivitis and arthralgia were reported in the northeastern Brazil. Although all patients lived in a dengue endemic area, molecular and serological diagnosis for dengue resulted negative. Chikungunya virus infection was also discarded. Subsequently, Zika virus (ZIKV) was detected by reverse transcription-polymerase chain reaction from the sera of eight patients and the result was confirmed by DNA sequencing. Phylogenetic analysis suggests that the ZIKV identified belongs to the Asian clade. This is the first report of ZIKV infection in Brazil.


Subject(s)
Adolescent , Adult , Aged , Animals , Female , Humans , Male , Middle Aged , Young Adult , Aedes/virology , Insect Vectors/virology , Zika Virus Infection/transmission , Zika Virus/genetics , Base Sequence , Brazil/epidemiology , Phylogeny , Polymerase Chain Reaction , RNA, Viral , Sequence Analysis, DNA , Zika Virus Infection/epidemiology , Zika Virus Infection/virology , Zika Virus/isolation & purification
2.
Arq. bras. oftalmol ; 73(3): 235-239, jun. 2010. graf, tab
Article in Portuguese | LILACS | ID: lil-555063

ABSTRACT

Objetivos: Este trabalho teve o objetivo de identificar possível associação entre conjuntivite alérgica e infecção por Chlamydia trachomatis. Método: Realizamos um estudo prospectivo em 104 olhos de 52 pacientes com hipótese diagnóstica de conjuntivite alérgica primaveril e atópica. Os pacientes foram examinados no Núcleo Especializado em Oftalmologia de João Pessoa - NEO. Foi realizada em todos os 52 pacientes, a citologia conjuntival, uma vez que pode evidenciar a presença de eosinófilos e corpúsculos de inclusões no esfregaço do raspado conjuntival, como também a imunofluorescência direta por ser o exame de escolha para confirmação de infecção por Chlamydia trachomatis. Todos os procedimentos foram aprovados pelo Comitê de Ética em pesquisa. Resultados: Dos 52 pacientes, 41 (78,8 por cento) apresentou conjuntivite primaveril e 11 (21,2 por cento) conjuntivite atópica. Quarenta e um (78,8 por cento) eram do sexo masculino e 11 (21,2 por cento) do feminino. As idades variaram de 3 a 19 anos, com uma média de 9,8 anos. Quanto à distribuição racial, 16 (30,8 por cento) pacientes eram brancos, 14 (26,9 por cento) eram negros e 22 (42,3 por cento) eram pardos. Pacientes com doença alérgica sistêmica observou-se que 25 (48,1 por cento) pacientes apresentaram asma brônquica, 20 (38,5 por cento) rinite alérgica e 5 (9,6 por cento) dermatite atópica. Os principais sintomas relatados pelos pacientes foram coriza (59,6 por cento), prurido ocular (98,1 por cento), ardor ou queimação (61,5 por cento), lacrimejamento (65,3 por cento) e fotofobia (61,5 por cento). Os principais sinais clínicos foram: hiperemia ocular (100 por cento), bilateralidade (100 por cento), papilas no tarso (92,3 por cento) e secreção mucosa (82,7 por cento). A citologia do raspado conjuntival encontrou o eosinófilo em 86,5 por cento dos casos. Apenas 7 pacientes não apresentaram eosinófilos no raspado conjuntival. Observamos que 3 pacientes (5,8 por cento) apresentou imu...


Purpose: The aim of this paper was to identify a possible association between allergic conjunctivitis and Chlamydia trachomatis infection. Methods: A prospective study was carried out in 104 eyes of 52 patients with diagnosis of vernal conjunctivitis and atopic keratoconjunctivitis. Conjunctival cytology was performed in all the 52 patients, once it can evidence eosinophils presence and inclusions corpuscles in the conjunctival scraping smear, as well as the direct immunofluorescence that is the choice exam for Chlamydia trachomatis infection confirmation. All procedures were approved by the Institutional Review Board Ethics Committee. Results: Of the 52 patients, 41 (78.8 percent) presented vernal conjunctivitis and 11 (21.2 percent) atopic keratoconjunctivitis. Forty-one patients (78.8 percent) were male and 11 (21.2 percent) female. The ages varied from 3 to 19 years, with an average of 9.8 years. Regarding racial distribution, 16 (30.8 percent) patients were caucasian, 14 (26.9 percent) were black and 22 (42.3 percent) were brown. Regarding systemic allergic diseases, it was observed that 25 (48.1 percent) patients presented asthma, 20 (38.5 percent) allergic rhinitis and 5 (9.6 percent) atopic dermatitis. The main symptoms complained by the patients were coryza (59.6 percent), ocular itching (98.1 percent), burning (61.5 percent), tearing (65.3 percent) and photophobia (61.5 percent). The main clinical signs were: ocular hyperemia (100 percent), bilaterality (100 percent), superior tarsal papilla (92.3 percent) and mucous secretion (82.7 percent). The cytology of the conjunctival scrapings found eosinophil in 86.5 percent of the cases. Only seven patients did not present eosinophils in the conjunctival scrapings. Three patients (5.8 percent) presented positive immunofluorescence for Chlamydia, evidencing an association between allergic conjunctivitis and trachoma. Conclusion: It is important to determine the simultaneous coexistence ...


Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Young Adult , Chlamydia trachomatis/isolation & purification , Conjunctivitis, Allergic/complications , Trachoma/complications , Conjunctivitis, Allergic/diagnosis , Fluorescent Antibody Technique, Direct , Prospective Studies , Trachoma/diagnosis , Young Adult
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